A natural transformation is a mapping between two functors. The functors must have the same domain and codomain as each other. There is also the natuality square condition that must apply for this to be a natural transformation as we shall see below. 
Components
For components we will be working in terms of internal objects:
ComponentsFirst we take an object 'x' in 'C' and we see that this maps to a morphism in 'D'. For every object 'x' in C there is a morphism in D (f x > g x) known as the components of α at x written α_{x}. 

Natuality SquareSo now we know what happens to on object in 'C', we now want to know what happens to structure in 'C', for this we see what happens to a morphism in 'C'. A morphism in C would map to two component morphisms in D. This diagram must commute for every morphism in C. So α_{x} is the component of the natural transform at x and α_{y} is the component of the natural transform at y. For naturality we require that the square in D commutes, that is, α_{y}• M = N • α_{x} where: M = F m so: α_{y}• F m = G m • α_{x} 

Here is a more schematic view of the For a natural transformation α: F > G 

2CategoriesWe could generalise this to any structure in C which would have two components in D. Usually we define categories from the outside using arrows, in this case we look 1 level inside the category, two layers of category are known as a 2category. 

Composition VerticalWe can apply α to F to get G and then β to get H. 

Composition Horizontal

Horizontal and Vertical Composition
So a natural transformation adds another layer on top of functors. When we looked at functors we saw that we can see them at two levels, we can see them in terms of internal objects, or we can 'lift' the view to be in terms of structure.
So how can functors of functors occur? That is functors that map one functor to another, there are at least two ways which I will show here as horizontal or vertical:
Horizontal  The first way that we can get functors of functors is not new, it arises from morphisms. A function of components (x and y) gives rise to a function of the function 'h'.  
Vertical  The natural transform is shown here as a vertical double arrow. We can 'lift' the view, so that the natural transformation relates the functors F & G in a way that is independent of the underlying categories C & D. So we have a morphism of functors, that is we can regard the functors as objects and α as a functor. 

Square (combination of horizontal and vertical)  The natuality square requirement combines these vertical and horizontal functors into to a square which must commute for naturality. α_{y} º F(h) = G(h) º α_{x} 
or if we notate: β_{F} = F(h) and β_{G}= G(h):
α_{y} º β_{F} = β_{G} º α_{x}
So now we have 3 levels at which we can view natural transformations:
 0  Lowest level  In terms of internal objects
 1  Mid level  In terms of structure of original categories
 2  Highest level  Treating functors as 'objects' and natural transformations as functors.
When working at level 1 and 2 we must remember that the natuality square (see below) applies but when we include the lowest level natuality comes out of the diagram automatically, so we will start by working in terms of the internal objects, until the natuality square becomes intuitive.
Examples
Example  Computer Language Types  Lists
On the functor page we saw how an example of lists (as used in computer languages) could be seen as instances of functors, here we extend that example to natural transformations.
If we have a functor: C > List (C) then we can define a natural transformation 'reverse' which reverses the order of the list. This is defined in a way that is independent of whether it is a list of integers, booleans or whatever.  
Natuality square for List(Int) 
In the case of containers like this:
 Functor (map) does not change container structure but needs to be able to operate on elements.
 Natural transformations change the container structure but do not require a knowledge of the internal structure of elements.
A list may also be thought of as a cartesian product.
Take example 'C' is a set containing elements such as x and y. 'D' contains lists written as [x] for a list with a single element x.
The carteasian product of x1 and x2 in set is mapped to a list containg x1 and x2.
Example 2  Permutation Groups
As our category with the object being a set and the morhisms being the permutations of the set elements. We can think of composition of these permutations as being a natural transformation. See Yonada lemma. 
Proof
On site here are definitions in Isabella.